apc anti ulbp2 5 6  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor Cells

doi: 10.3389/fimmu.2019.00569

Figure Lengend Snippet: H3K9 acetylation-associated memory phenotype of γδ T cells upon in vitro co-culture with tumor cells. γδ T cell lines generated from 12 days zoledronic acid-stimulated PBMC were co-cultured with 2.5 mM VPA pre-treated Panc89 and PC-3 cells. After 24 h of co-culture, cells were harvested and stained with FITC-conjugated Vδ2, APC-H7-conjugated CD27, PE-Cy7-conjugated CD45RA and Pacific Blue-conjugated H3K9ac antibodies. Based on flow cytometry gating strategy described in the , Vδ2-gated cells were defined as (A) H3K9ac high and (B) H3K9ac low in solo- or co-culture with Panc89 and PC3 cells in the presence and absence of 2.5 mM VPA. The proportions of H3K9ac low cells within Vδ2 T cells with (C) T EM and (D) T EMRA phenotype from the co-culture with Panc89 or PC-3 with or without 2.5 mM VPA treatment are shown. Data represented as mean ± S.E. of four independent experiments. Statistical significance is shown by * , ** , *** for p -values < 0.05, 0.01 and 0.005, respectively.

Article Snippet: Cells were harvested, washed and surface stained with the following anti-human antibodies: PE-conjugated anti-human MICA (clone 159227; #FAB1300P), Alexa Fluor 700-conjugated anti-human MICB (clone 236511, #FAB1599N), PerCp-conjugated anti-human ULBP-1 (clone 170818, #FAB1380C) and APC-conjugated anti-human ULBP2/5/6 (clone 165903, #FAB1298A) together with respective isotype controls (all from R&D Systems).

Techniques: In Vitro, Co-Culture Assay, Generated, Cell Culture, Staining, Flow Cytometry